Table 2 Averaged values of key process parameters obtained by fed-batch cultivations. Nominal and experimental specific growth rate (µ), maximum specific growth rate (µmax), final product titer, final dry cell weight (DCW), substrate consumption rate (qS), specific CalB production rate (qP), biomass-to-substrate yield (YX/S), product-to-biomass yield (YP/X) and product-to-substrate yield (YP/S). Results represent the average of two biological replicates. ± indicate SD
| Â | Integrative | Episomal | Increase |
|---|---|---|---|
Nominal µ (h− 1) | 0.05 | 0.05 | - |
Experimental µ (h− 1) | 0.0466 ± 0.0028 | 0.0472 ± 0.0028 | + 1.4% |
Final product titer (AU · L− 1) | 4756 ± 64 | 9906 ± 463 | + 108.3% |
Final DCW (g L− 1) | 77.18 ± 1.16 | 79.70 ± 1.24 | + 3.3% |
qS(gS· gX−1· h− 1) | 0.0898 ± 0.0067 | 0.0893 ± 0.0008 | - 0.56% |
qP (AU gX−1 h− 1) | 3.36 ± 0.33 | 7.13 ± 0.54 | + 112.4% |
YX/S(gX· gS−1) | 0.519 ± 0.012 | 0.529 ± 0.027 | + 1.9% |
YP/X(AU · gX−1) | 72.04 ± 5.73 | 150.87 ± 7.07 | + 109.4% |
YP/S(AU · gS−1) | 45.96 ± 3.17 | 91.59 ± 3.11 | + 99.3 |