Scalable protein production by Komagataella phaffii enabled by ARS plasmids and carbon source-based selection

Table 1 All plasmids generated in this study

Plasmid Number	Description	Selection marker	Name	Promoter	PARS1
1	Cloning vectors; not used in K. phaffii	GUT1/Ampicillin	pBSY3G	P_GUT1	-
2			pBSY3G_PARS1	P_GUT1	+
3			pBSY3G_DαCalB	P_GUT1	-
4			pBSY3G_PARS1_DαCalB	P_GUT1	+
5	K. phaffii expression vectors	GUT1	pBSY3G_DαCalB_P_AgTEF1	P_AgTEF1	-
6			pBSY3G_PARS1_DαCalB_P_AgTEF1	P_AgTEF1	+
7			pBSY3G_DαCalB_P_GCW14	P_GCW14	-
8			pBSY3G_PARS1_DαCalB_P_GCW14	P_GCW14	+
9			pBSY3G_DαCalB_P_GUT1	P_GUT1	-
10			pBSY3G_PARS1_DαCalB_P_GUT1	P_GUT1	+
11			pBSY3G_DαCalB_P_ILV5	P_ILV5	-
12			pBSY3G_PARS1_DαCalB_P_ILV5	P_ILV5	+
13			pBSY3G_DαCalB_P_TPI1	P_TPI1	-
14			pBSY3G_PARS1_DαCalB_P_TPI1	P_TPI1	+
15	K. phaffii expression vectors	TPI1	pBSY3T_DαCalB_P_AgTEF1	P_AgTEF1	-
16			pBSY3T_PARS1_DαCalB_P_AgTEF1	P_AgTEF1	+
17			pBSY3T_DαCalB_P_GCW14	P_GCW14	-
18			pBSY3T_PARS1_DαCalB_P_GCW14	P_GCW14	+
19			pBSY3T_DαCalB_ P_GUT1	P_GUT1	-
20			pBSY3T_PARS1_DαCalB_ P_GUT1	P_GUT1	+
21			pBSY3T_DαCalB_P_ILV5	P_ILV5	-
22			pBSY3T_PARS1_DαCalB_P_ILV5	P_ILV5	+
23			pBSY3T_DαCalB_P_TPI1	P_TPI1	-
24			pBSY3T_PARS1_DαCalB_P_TPI1	P_TPI1	+

ISSN: 1475-2859