Fig. 4
Re-screening of individual cells from bioreactor cultivation. Sample 8 from the bioreactor cultivations were plated on BMG1 agar plates to obtain single colonies. A 96 deep-well plate containing 250 µl BMG1 medium was inoculated with one single colony per well. After 60 h of incubation at 28 °C and 320 rpm, 250 µl of BMG0.5 were added, followed by 50 µl BMG2.5 after 72 h and 84 h to allow de-repression of PDC and consequent reporter gene expression. Cultivations were harvested after 108 h of cultivation by centrifugation and CalB activities in the supernatants were evaluated. Each bar represents a single well of the deep-well plate