Fig. 2
CalB reporter activities from 250 mL shake flask cultivations. Five transformants of ARS plasmids with PAgTEF1 driving the GUT1 or TPI1 marker expression were used to directly inoculate baffled 250 mL shake flasks containing 50 mL of BMG1. The respective average, integrative controls were cultivated in triplicates. After 60 h, 72 h and 84 h GUT1 plasmid cultivations and after 100 h, 112 h and 124 h TPI1 plasmid cultivations were fed with 500 µL of 50% glycerol. After 108 h the GUT1 plasmid cultivations and after 148 h the TPI1 plasmid cultivations were harvested and CalB activities were measured in the supernatants