Fig. 1
CalB reporter activities from transformants of episomal plasmid variants cultivated in micro-scale deep-well plates. Seven transformants of various ARS plasmids with different combinations of selection marker and its promoter, together with 7 colonies of the respective integrative references, were cultivated in 96 deep-well plates containing 250 µl BMG1. After 60 h of incubation at 28 °C and 320 rpm, 250 µl of BMG0.5 were added, followed by 50 µl BMG2.5 after 72 and 84 h to allow de-repression of PDC and consequent reporter gene expression. Cultivations were harvested after 108 h of cultivation by centrifugation and CalB activities in the supernatants were evaluated