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Fig. 4 | Microbial Cell Factories

Fig. 4

From: High cell density sequential batch fermentation for enhanced propionic acid production from glucose and glycerol/glucose mixture using Acidipropionibacterium acidipropionici

Fig. 4

Sequential batch fermentation of glycerol/glucose mixture for production of propionic acid using A. acidipropionici DSM 4900 with cell recycle. Fermentation temperature was maintained at 32 °C, pH at 6.5 by the addition of 5N NH4OH solution. A The concentrations of glucose (black square), glycerol (×) and propionic acid (black diamond), B the concentration of succinic acid (Black circle) and acetic acid (black triangle), C the concentration of biomass (+), and D the microbial growth shown as Ln(CDW) (–).The production medium contained ~ 30 g/L glucose, 60 g/L glycerol, and 1.25× heat-treated potato juice. The medium was supplemented with 0.5 mg/L biotin (batch 7), or 0.75 mg/L biotin (batches 8, 9 and 10)

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