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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Genetic toolbox for Photorhabdus and Xenorhabdus: pSEVA based heterologous expression systems and CRISPR/Cpf1 based genome editing for rapid natural product profiling

Fig. 3

Overview of the developed single plasmid CRISPR-Cpf1 genome editing workflow. The approach starts with conjugation of the target specific pAR20 into the strain to be edited (day 0); selection of conjugates and induction of CRISPR/Cpf1 components (day 3); plasmid curing of correct edited strains with sucrose (day 5); screening for new or known compounds (day 6); entering a new editing cycle (day 7). (Km = kanamycin; L-Ara = L-Arabinose; ATC = anhydrotetracycline). Figure created with BioRender.com

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Microbial Cell Factories

ISSN: 1475-2859

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