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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Genetic toolbox for Photorhabdus and Xenorhabdus: pSEVA based heterologous expression systems and CRISPR/Cpf1 based genome editing for rapid natural product profiling

Fig. 2

Safracin cluster activation and refactoring in Xenorhabdus sp. TS4. A) Constructed safracin producer strains, I represents the wildtype strain, IIa promoter exchange strain with Pvan/PproC insertion, IIb Pvan/PproD insertion and strain II with additional Ptac insertion upstream of xscK with xscH deletion. B) Putative biosynthesis of safracins; tailoring enzyme modifications are highlighted; Tyr* indicates the specificity of the XscC A domain for 3-hydroxy-5-methyl-o-methyltyrosine; “Unnatural” further modification of SAC-B by SfmCy resulting in 24. C) Comparison of SAC-A and SAC-B production between the constructed strains. A: adenylation domain, C: condensation domain, R: reduction domain, PCP: peptidylcarrier protein (small black circle)

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Microbial Cell Factories

ISSN: 1475-2859

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