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Fig. 6 | Microbial Cell Factories

Fig. 6

From: Harnessing alkaline-pH regulatable promoters for efficient methanol-free expression of enzymes of industrial interest in Komagataella Phaffii

Fig. 6

An evolved pPHO89-phytase strain surpasses the production levels of the pAOX1-phytase strain. (A) Three evolved pPHO89-phytase strains (J5, J6 and J7), derived from pP89-J, were tested for phytase production as shown in Fig. 2A. Data are presented as the mean ± SEM from 3 to 5 independent cultures for each strain. (B) The production level of parental pP89-J and the three evolved strains was compared for each time point with that of the pAOX1-based strain, taken as the unit. *, p < 0.05; **, p < 0.01; *** p < 0.005. (C) SDS‒PAGE analysis of the medium recovered from cultures of the pAOX1-based and the evolved pP89-J6 strain. Samples corresponding to 3.75 µl of medium were run on 10% polyacrylamide gels and stained with Coomassie Blue. Specific quantities (1 and 3 µg) of bovine serum albumin (BSA) were included in the gel for comparison of the amounts of expressed proteins. M denotes molecular mass standards

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Microbial Cell Factories

ISSN: 1475-2859

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