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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Harnessing alkaline-pH regulatable promoters for efficient methanol-free expression of enzymes of industrial interest in Komagataella Phaffii

Fig. 3

Mapping the pH-dependent activation of the TSA1 promoter. A new ser of yeast strains that contained the indicated versions of the TSA1 promoter driving the expression of phytase were created (panel A). The location of predicted transcription factor-binding sites with a p value < 2 × 10− 5 is denoted at the bottom of the cartoon. Cells were grown on YPGly and tested for phytase activity after 48 h of induction (no restoration of pH or nutrients) as indicated in Material and Methods (B and C). Data are presented as the mean ± SEM from 4 (non-induced) or 8 (induced) independent experiments

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Microbial Cell Factories

ISSN: 1475-2859

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