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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Harnessing alkaline-pH regulatable promoters for efficient methanol-free expression of enzymes of industrial interest in Komagataella Phaffii

Fig. 2

Alkali-induced production of phytase from the TSA1 promoter. (A) Three independent clones containing an integrated pTSA1-phytase construct were grown on YP with glycerol as the carbon source and processed as described in the Materials and Methods. Cells were shifted to pH 8.0 at time 0 (or maintained at pH 5.5), and cultures were subjected to periodic restoration of the pH and carbon source. Samples were taken at the indicated times, and phytase activity was determined after appropriate dilution with water. The activity produced by a pAOX1-phytase strain grown on YP medium is included for comparison. Data are presented as the mean ± SEM from 3 to 5 independent cultures for each clone. (B) The pTSA1-1 clone was processed as in panel A except that cells were shifted at the indicated pH values. Data are the mean ± SEM from 3 independent cultures

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Microbial Cell Factories

ISSN: 1475-2859

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