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Table 2 Funding this research factors influencing cellulase production by the fusant (PFNT) using solid substrate SCB and WB combined in a ratio of 1:1 as carbon source

From: Enhancement of cellulolytic enzyme production from intrageneric protoplast fusion of Aspergillus species and evaluating the hydrolysate scavenging activity

Trials

Variables

Cellulase (U/ml)

X1

X2

X3

X4

X5

X6

X7

Experimental

Predicted

Residual

1

-1

-1

-1

1

-1

-1

1

1.592715

1.807955

-1.09599

2

1

1

1

-1

-1

-1

1

3.710501

3.433152

1.412246

3

-1

1

-1

1

1

1

-1

1.367739

1.090389

1.412246

4

-1

1

1

1

-1

-1

-1

1.92526

2.032159

-0.54432

5

1

1

1

1

1

1

1

1.977767

2.166343

-0.96022

6

-1

-1

1

-1

-1

1

-1

2.669111

2.535549

0.680089

7

-1

-1

1

-1

1

1

1

1.592715

1.726277

-0.68009

8

1

-1

-1

1

-1

1

1

2.966651

2.751411

1.095987

9

1

1

-1

-1

-1

1

-1

2.996641

3.300654

-1.54802

10

-1

1

-1

-1

1

-1

1

1.592715

1.547927

0.228061

11

1

-1

-1

-1

1

-1

-1

2.327815

2.34594

-0.09229

12

1

-1

1

1

1

-1

-1

2.039026

2.02090

0.092292

Variables

Code

Coded level and actual level

    
 

-1

1

      

SCB (gm)

X1

2

4

      

WB (gm)

X2

2

4

      

Moisture (%)

X3

60

80

      

pH (value)

X4

4

6

      

Temp. (°C)

X5

30

37

      

Time (days)

X6

4

8

      

Inoculum size (sp/ml)

X7

1 × 106

3 × 106

     Â