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Fig. 5 | Microbial Cell Factories

Fig. 5

From: PySupercharge: a python algorithm for enabling ABC transporter bacterial secretion of all proteins through amino acid mutation

Fig. 5

Secretion of supercharged botulinum toxin type A. (a) Top graph: LCD analysis result of BoNT wildtype sequence (cationic supercharged regions of LCD > 2 shaded green). Middle graph: Mutated sequence of BoNT (LCD ≤ 2). Note that all the shaded regions from the top graph have been modified to stay below or on the red LCD = 2 reference line. LCD > 1 regions have been shaded in red for easy comparison with BoNT (LCD ≤ 1). Bottom graph: Mutated sequence of BoNT (LCD ≤ 1). Note that all the shaded regions from the top graph have been modified to stay below or on the green LCD = 1 reference line. (b) BoNT/A wildtype protein structure with residues mutated in BoNT (LCD ≤ 2) highlighted in red. (c) The western blot analysis results of BoNT. Supercharged BoNT (LCD ≤ 2) and BoNT (LCD ≤ 1) clearly display higher secretion ability, the latter being even higher than the former

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Microbial Cell Factories

ISSN: 1475-2859

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