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Fig. 3 | Microbial Cell Factories

Fig. 3

From: PySupercharge: a python algorithm for enabling ABC transporter bacterial secretion of all proteins through amino acid mutation

Fig. 3

Secretion results of manually supercharged human growth factors. (a) LCD analysis results of wildtype sequences (shown in grey) and manually supercharged sequences (shown in red) of various human growth factors. Each plotted point matches an amino acid position to the LCD value of the 20-amino-acid window starting from that position. (b) Adapted from “Utilizing the ABC Transporter for Growth Factor Production by fleQ deletion mutant of Pseudomonas fluorescens”, Fabia et al., Biomedicines 2022, 9(6), p. 679 [2]. Adapted with permission- label sizes and colors have been modified. The western blotting results of manually mutated growth factors (bottom row) show enhanced secretion ability compared to the wildtype proteins (top row). A separate high-exposure western blot of wildtype IGF1 and IGF2 is in an additional file [see Additional File 4] due to low visibility in this figure

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Microbial Cell Factories

ISSN: 1475-2859

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