Strains | Descriptions |
---|---|
Host strains | |
Escherichia coli JM109 | wild-type E. coli |
Pichia pastoris GS115 | wild-type Pichia pastoris |
H001 | GS115 mutant in PAS_chr1-3_0225 gene |
H002 | GS115 mutant in PAS_chr2-1_0661 gene |
GFP-expressing strains | |
GS115-pGAPZ A-gfp | GS115 harboring gfp under PGAP |
GS115-pPICZ A-gfp | GS115 harboring gfp under PAOX |
H001-pGAPZ A-gfp | H001 harboring gfp under PGAP |
H002-pGAPZ A-gfp | H002 harboring gfp under PGAP |
H001-pPICZ A-gfp | H001 harboring gfp under PAOX |
H002-pPICZ A-gfp | H002 harboring gfp under PAOX |
hegf-expressing strains | |
GS115-pGAPZα A-hegf | GS115 harboring hegf under PGAP and α factor |
GS115-pPICZα A-hegf | GS115 harboring hegf under PAOX and α factor |
H001-pGAPZα A-hegf | H001 harboring hegf under PGAP and α factor |
H002-pGAPZα A-hegf | H002 harboring hegf under PGAP and α factor |
H001-pPICZα A-hegf | H001 harboring hegf under PAOX and α factor |
H002-pPICZα A-hegf | H002 harboring hegf under PAOX and α factor |
SAM-biosynthesis strains | |
GS115-pGAPZ A-sam2 | GS115 harboring sam2 under PGAP |
GS115-pPICZ A-sam2 | GS115 harboring sam2 under PAOX |
H001-pGAPZ A-sam2 | H001 harboring sam2 under PGAP |
H002-pGAPZ A-sam2 | H002 harboring sam2 under PGAP |
H001-pPICZ A-sam2 | H001 harboring sam2 under PAOX |
H002-pPICZ A-sam2 | H002 harboring sam2 under PAOX |
ERG-biosynthesis strains | |
GS115-pGAPZ A-egt12 | GS115 harboring egt12 under PGAP |
GS115-pGAPZ A-egt1E | GS115 harboring egt1E under PGAP |
GS115-pPICZ A-egt12 | GS115 harboring egt12 under PAOX |
GS115-pPICZ A-egt1E | GS115 harboring egt1E under PAOX |
H001-pGAPZ A-egt12 | H001 harboring egt12 under PGAP |
H001-pGAPZ A-egt1E | H001 harboring egt1E under PGAP |
H001-pPICZ A-egt12 | H001 harboring egt12 under PAOX |
H001-pPICZ A-egt1E | H001 harboring egt1E under PAOX |
H002-pGAPZ A-egt12 | H002 harboring egt12 under PGAP |
H002-pGAPZ A-egt1E | H002 harboring egt1E under PGAP |
H002-pPICZ A-egt12 | H002 harboring egt12 under PAOX |
H002-pPICZ A-egt1E | H002 harboring egt1E under PAOX |