Fig. 5From: Recombinant L. lactis vaccine LL-plSAM-WAE targeting four virulence factors provides mucosal immunity against H. pylori infectionAntiserum specificity test for LL-plSAM-WAE. Antisera of mice induced by LL-plSAM-WAE via oral vaccination were collected to detect anti-H. pylori neutralizing antibodies. (a) The schedule of vaccination of BALB/c mice. (b) Western blot results showed that the antiserum obviously recognized the antigens Urease, UreA, UreB, HpaA and NAP. (c) Before ELISA, the antiserum was diluted to a concentration of 1:500, and the plates were coated with 5 µg/mL concentrations of SAM-WAE, urease, HpaA, HSP60, NAP and BSABack to article page