Fig. 1
From: Development and characterization of a novel nanobody with SRMV neutralizing activity
Serum antibody titer after immunization and biopanning process monitoring. (A) Serum antibody titer against SRMV-F and –H after immunization procedure, which were both up to 1:50000. (B) Enrichment evaluation of phage-displayed nanobodies by biopanning. Phage-displayed nanobodies were selected against SRMV-F and –H protein respectively, and E. coli TG1 was infected with bound phage. The number of phages eluted after each round of biopanning was counted based on the number of plaques (PFU/mL) formed after infection of the host bacteria with the eluted phage particles. (C) ELISA assay for selection of individual phage particles against SRMV-F and -H protein, respectively