Fig. 2From: Identification of squalene epoxidase in triterpenes biosynthesis in Poria cocos by molecular docking and CRISPR-Cas9 gene editingConstruction of Poria cocos squalene epoxidase gene knockout plasmid vector. A pFC332 empty plasmid. B A complete pFC332-U6-sgRNAPcSE-Cas9 knockdown vector containing two sgRNA loci was successfully constructed. C The fragment and its amino acid sequence were inserted into the construct vectorBack to article page