Fig. 5
Verification of genetic accessibility of the type strains of E. limosum (DSM 20543T) and E. callanderi ‘FD’ (DSM 3662T), as well as the strains ‘Marburg’ (DSM3468), ‘KIST612’, ‘2A’ (DSM 2593), ‘11A’ (DSM 2594), ‘G14’ (DSM 107592), and ‘32’ (DSM 20517) based on the FAST-mediated fluorescence. A Fluorescence intensities of the whole cell populations of recombinant strains were determined using a microplate reader in presence of the fluorogen TFLime. Successfully transformed strains resulted in bright fluorescence. Strains harboring the empty vector control were non-fluorescent. Strain ‘G14’ was not transformed and was therefore non-fluorescent. B Number of fluorescent cells of the recombinant strains determined using flow cytometry. C Density plots of recombinant DSM 3662T [pJIR751] and DSM 3662T [pJIR751_Pfd_FAST] cells. Expression of feg resulted in a heterogeneous population of DSM 3662.T [pJIR751_Pfd_FAST]. Error bars indicate standard deviations. n = 3