Strains or plasmids | Description | Source or reference |
---|---|---|
Strains | ||
 E. coli | ||
  JM83 | F’, ara, Δ(lac-pro AB), rpsL, (Strr), Φ80, lacZΔM15 | [27] |
  ET12567/pUZ8002 | dam-13::Tn9 dcm-6 hsdM; containing the non-transmissible RP4 derivative plasmid pUZ8002 | [27] |
 S17-1 | recA, pro, hsdR, RP4-2-Tc::Mu-Km::Tn7 | [37] |
 S. lincolnensis | ||
  NRRL 2936 | Wild-type (WT), lincomycin producer | NRRL, USA |
  ΔlmbU | NRRL 2936 with in-frame deletion of lmbU | This study |
  WT/p0469TE | NRRL 2936 attBφC31::p0469TE | This study |
  ΔlmbU/p0469TE | ΔlmbU attBφC31::p0469TE | This study |
  WT/p1037TE | NRRL 2936 attBφC31::p1037TE | This study |
  ΔlmbU/p1037TE | ΔlmbU attBφC31::p1037TE | This study |
  WT/p8097TE | NRRL 2936 attBφC31::p8097TE | This study |
  ΔlmbU/p8097TE | ΔlmbU attBφC31::p8097TE | This study |
  ΔSLINC_0469 | NRRL 2936 with in-frame deletion of SLINC_0469 | This study |
  ΔSLINC_1037 | NRRL 2936 with in-frame deletion of SLINC_1037 | This study |
ΔSLINC_8097 | NRRL 2936 with in-frame deletion of SLINC_8097 | This study |
  CSLINC_0469 | SLINC_0469 complementation strain | This study |
  CSLINC_1037 | SLINC_1037 complementation strain | This study |
  CSLINC_8097 | SLINC_8097 complementation strain | This study |
Plasmids | ||
 pLU-1 | lmbU cloned in NdeI/EcoRI sites of pET-28a ( +), for LmbU expression | [27] |
 pKCcas9dO | actII-orf4-specific guide-RNA, homologous arms flanking actII-orf4, aac(3)IV, pSG5 | [41] |
 pKCcas9dlmbU | lmbU-specific guide-RNA, homologous arms flanking lmbU, aac(3)IV, pSG5 | This study |
 pIB139 | Integrative vector based on φC31 integrase, ermE*p | [27] |
 pPU139 | pIB139 carrying lmbU gene under its own promoter | This study |
 pATE152 | pSET152 derivative carrying xylTE gene under lmbAp promoter | [42] |
 pSET152 | Integrative vector based on φC31 integrase | [43] |
 p0469TE | pSET152 carrying xylTE gene under the control of SLINC_0469p | This study |
 p1037TE | pSET152 carrying xylTE gene under the control of SLINC_1037p | This study |
 p8097TE | pSET152 carrying xylTE gene under the control of SLINC_8097p | This study |
 pKCcas9d0469 | SLINC_0469-specific guide-RNA, homologous arms flanking SLINC_0469, aac(3)IV, pSG5 | This study |
 pKCcas9d1037 | SLINC_1037-specific guide-RNA, homologous arms flanking SLINC_1037, aac(3)IV, pSG5 | This study |
 pKCcas9d8097 | SLINC_8097-specific guide-RNA, homologous arms flanking SLINC_8097, aac(3)IV, pSG5 | This study |