Fig. 2

Observations of S. cerevisiae cells and isolated vacuoles. a Cell pellet of the vector control strain and the strain expressing Avt6-dR supplemented with all-trans-retinal. b Fluorescence microscopy images of cells expressing GFP (upper panels) and Avt6-dR-GFP (lower panels). The vacuolar membrane was labeled with FM4-64. c Vacuoles isolated from the vector control strain and the strain expressing Avt6-dR. d Fluorescence microscopy images of vacuoles isolated from the vector control strain and the strain expressing Avt6-dR. The vacuolar membrane was labeled with FM4-64 (magenta). Each scale bar (yellow line) represents 5 µm