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Fig. 8 | Microbial Cell Factories

Fig. 8

From: Selective extraction of recombinant membrane proteins from Hansenula polymorpha by pulsed electric field and lytic enzyme pretreatment

Fig. 8

SDS PAGE (A) and Western blot (B) of the proteins released from PEF treated cells after incubation with lyticase and the following treatment with detergent. (A) Lane M, molecular weight marker; lane 1, proteins released from PEF treated cells after 2 h incubation with 20 U mL− 1 lyticase and 5 mM DTT; lane 2, proteins released during incubation with 1% Triton X-100 (75 min, 39 oC); lane 3 extracts obtained from cells preincubated for 10 min at 60 oC and subsequently incubated with Triton X-100 at 39 oC. The thermostable host protein is indicated with asterisk. (B) Lane M, molecular weight marker; lane 1, supernatant obtained after treatment with enzyme; lane 2, insoluble fraction obtained after treatment with Triton X-100; lane 3, soluble fraction (A, lane 2) obatined after treatment with Triton X-100

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Microbial Cell Factories

ISSN: 1475-2859

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