Prediction of BMI traits in the Chinese population based on the gut metagenome

Table 1 Overview of data included in the study

Study ID	Age(years) Mean ± SD	Sex	BMI (kg/m²) Mean ± SD	Stool Sample Collection Method and Storage	Sequencing Platform
Zeng2021 [31] DOI:https://doi.org/10.1099/mgen.0.000639	44.7 ± 9.1	93♀ 57♂	27.44 ± 2.25	Collected from sterile stool containers and frozen at -80℃ ⁽¹⁾	Illumina
Zhang 2015 [32] DOI:https://doi.org/10.1038/nm.3914	42.9 ± 8.4	49♀ 20♂	23.62 ± 3.53	Collected in hospital and stored at -80℃ ⁽²⁾	Illumina
Qin 2012 [33] DOI:https://doi.org/10.1038/nature11450	40.8 ± 12.8	46♀ 43♂	21.23 ± 3.17	Collected at home and frozen − 80℃ ⁽³⁾	Illumina

(1). During physical examination, fresh stools were collected from individuals using sterile stool containers. Approximately 5 g stool from each individual was obtained using swabs (Huachenyang Technology). The stool samples were preserved in stool collection tubes (Axygen), and then transferred to a − 80 °C refrigerator (DW-86L626; Haier) within half an hour.
(2). Fecal samples were collected at Peking Union Medical College Hospital, transported frozen, and extracted at BGI-Shenzhen as Qin 2012 [33] described.
(3). Fresh faecal samples were obtained at home, and samples were immediately frozen by storing in a home freezer for less than 1 day. Frozen samples were transferred to BGI-Shenzhen, and then stored at -80 °C until analysis.

ISSN: 1475-2859