Table 1 Plasmids and strains used in this study
Description | Source | |
|---|---|---|
Plasmids | ||
pSEVA88c1 | Replicative shuttle vector | [45] |
pSEVA88c1-BGC2 | pSEVA88c1 harboring homologous arms for BGC2 deletion | This study |
pSEVA88c1-BGC5 | pSEVA88c1 harboring homologous arms for BGC5 deletion | This study |
pSEVAUO-C42012 | Replicative shuttle vector harboring the nuclease cas9 | [42] |
pSEVAUO-C42012-Spacer-BGC2 | pSEVAUO-C42012 harboring the protospacer for BGC2 deletion | This study |
pSEVAUO-C42012-Spacer-BGC5 | pSEVAUO-C42012 harboring the protospacer for BGC2 deletion | This study |
pSEVAUO-C42012-BGC2 | pSEVAUO-C42012-Spacer-BGC2 harboring homologous arms for BGC2 deletion | This study |
pSEVAUO-C42012-BGC5 | pSEVAUO-C42012-Spacer-BGC5 harboring homologous arms for BGC5 deletion | This study |
pSEVAUO-M11701-NarBGC | Level 2 MoClo plasmid harboring TAL, 4CL, CHS and CHI | [42] |
PCR-Blunt II-TOPO | Replicative blunt DNA cloning vector | Invitrogen |
PCR-Blunt II-TOPO-OsNOMT | PCR-Blunt II-TOPO harboring OsNOMT | This study |
pSEVA181SF14 | Source of SF14 (Level 0 MoClo) | EXPLORA |
pSEVA181RiboJ-RBS | Source of RiboJ-RBS (Level 0 MoClo) | EXPLORA |
pIDTSMARTttsbib | Source of ttsbib (Level 0 MoClo) | IDT |
pSEVAUO-M21104 | Level 1 MoClo receptor | [42] |
pSEVAUO-M21104-OsNOMT | Level 1 MoClo harboring OsNOMT | This study |
PCR-Blunt II-TOPO-Pa4’OMT | PCR-Blunt II-TOPO harboring Pa4’OMT | This study |
PCR-Blunt II-TOPO-FNS1 | PCR-Blunt II-TOPO harboring FNS1 | This study |
pSEVAUO-M21102 | Level 1 MoClo receptor | [42] |
pSEVAUO-M21202-FNS1 | Level 1 MoClo harboring FNS1 | This study |
pSEVAUO-M21202 | Level 1 MoClo receptor | [42] |
pSEVAUO-M21102-Pa’4OMT | Level 1 MoClo harboring Pa4’OMT | This study |
pSEVAUO-M21503 | Level 2 MoClo receptor | [42] |
pSEVAUO-M21503-ACA | Level 2 MoClo plasmid harboring FNS1 and Pa4’OMT | This study |
pSEVAUO-M21503-GNK | Level 2 MoClo plasmid harboring FNS1 and OsNOMT | This study |
Strains | ||
E. coli TOP10 | Strain used for routine subcloning | Invitrogen |
E. coli ET12567/pUZ8002 | Strain used for conjugation | Life Science |
UO-FLAV-002 | S. albidoflavus strain used for BGC2 deletion | [42] |
UO-FLAV-003 | UO-FLAV-002 lacking the BGC2 | This study |
UO-FLAV-004 | UO-FLAV-003 lacking the BGC5 | This study |
UO-FLAV-003-NAR | UO-FLAV-003 harboring TAL, 4CL, CHS and CHI | This study |
UO-FLAV-004-NAR | UO-FLAV-004 harboring TAL, 4CL, CHS and CHI | This study |
UO-FLAV-004-API | UO-FLAV-004 harboring TAL, 4CL, CHS, CHI and FNS1 | This study |
UO-FLAV-004-SAK | UO-FLAV-004 harboring TAL, 4CL, CHS, CHI and OsNOMT | This study |
UO-FLAV-004-ACA | UO-FLAV-004 harboring TAL, 4CL, CHS, CHI, FNS1 and Pa4’OMT | This study |
UO-FLAV-004-GNK | UO-FLAV-004 harboring TAL, 4CL, CHS, CHI, FNS1 and OsNOMT | This study |
UO-FLAV-004-OsNOMT | UO-FLAV-004 harboring OsNOMT | This study |
UO-FLAV-004-FNS1 | UO-FLAV-004 harboring FNS1 | This study |