Identification and statistical optimization of a novel alginate polymer extracted from newly isolated Synechocystis algini MNE ON864447 with antibacterial activity

Table 3 Face central composite design for cyanobacterial alginate production from S. algini MNE ON864447 as influenced by working volume (X1), the incubation period (X2), and inoculum size (X3)

Std	Run	Factors			Total polysaccharide yield (g/L)		Residuals
		X1	X2	X3	Total polysaccharide yield (g/L)
		X1	X2	X3	Actual	Predicted
14	1	0	0	1.68179	1.88983	1.95	−0.0632
17	2	0	0	0	1.395	1.45	−0.0564
13	3	0	0	−1.68179	3.23105	3.14	0.0869
4	4	1	1	−1	1.90191	1.95	−0.0501
12	5	0	1.68179	0	0.97914	0.9839	−0.0048
9	6	−1.68179	0	0	2.1476	2.15	−0.0009
19	7	0	0	0	1.49155	1.45	0.0402
5	8	−1	−1	1	1.96726	1.93	0.0334
20	9	0	0	0	1.49672	1.45	0.0454
18	10	0	0	0	1.42431	1.45	−0.0271
2	11	1	−1	−1	2.14052	2.18	−0.0405
16	12	0	0	0	1.45672	1.45	0.0054
15	13	0	0	0	1.43983	1.45	−0.0115
11	14	0	−1.68179	0	1.64845	1.62	0.0284
1	15	−1	−1	−1	3.57431	3.63	−0.0533
8	16	1	1	1	2.26586	2.23	0.0366
6	17	1	−1	1	0.77397	0.7749	−0.0009
3	18	−1	1	−1	1.40105	1.42	−0.0158
10	19	1.68179	0	0	1.64845	1.62	0.0245
7	20	−1	1	1	1.43019	1.41	0.0238

Level	Working volume (mL)	The incubation period (days)	Inoculum size (%)
−1.68	300	15	2
−1	400	20	4
0	500	25	6
1	600	30	8
1.68	700	35	10

ISSN: 1475-2859