Fig. 2

Global comparison of RNA-seq data of XynA overexpressing B. subtilis cells. Data from secreting (Xyn) and control strains containing the empty plasmid (Emp) at logarithmic growth (3 h) and stationary growth (6 h) are compared. A Principal component analysis (PCA) of the two biological replicates. Emp3h and Emp6h are RNA-seq samples of the control strain containing empty plasmid sampled at 3 and 6 h of growth, while Xyn3h and Xyn6h are RNA-seq samples of the Xyna secreting strain. The largest sample variation is caused by different growth phases (PC1, 3 h vs. 6 h), and the difference between the timepoints in PC1 is consistent for both the Xyn and Emp samples. B, C Volcano plots of differentially expressed genes (DEGs) induced by XynA overexpression (BWB09/pXynA vs. BWB09/pEmp) at 3 h and 6 h. The dashed line indicates a p-value of 0.05. Significantly up- and downregulated genes (p-value < 0.05) are colored in red and green respectively. D Venn diagrams with the number of significant up- and downregulated genes (p-value < 0.05) affected by the stress of XynA secretion at 3 h (in blue) and 6 h (in yellow)