Fig. 2
From: A novel protein purification scheme based on salt inducible self-assembling peptides
Purification of LCB3/SpyCatcherΔN-ELP-SpyCatcherΔN/xylanase with 0.7 M Na2SO4. M1 and M2: protein marker. EP: precipitate of the cell lysate after expression, ES: supernatant of the cell lysate after expression, ESS, supernatant of salt-induced aggregation, ESP: precipitate of salt-induced aggregation, CP: precipitate of intein-mediated cleavage, CS and 5×CS: supernatant of intein-mediated cleavage, the 5×CS was loaded at 5 times the amount of the CS. a LCB3. b SpyCatcherΔN-ELP-SpyCatcherΔN. c-d Purification results for xylanase from the two fusion proteins, MpA-Mtu ΔI-CM-xylanase (c) and xylanase-Mxe GyrA-MpA (d), individually