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Table 1 Determination of error rate values for IME199 DNAP during MDA

From: Identification of a novel family B DNA polymerase from Enterococcus phage IME199 and its overproduction in Escherichia coli BL21(DE3)

Experiment

mf

d

ER

1

6.9 × 10−3

6.78

3.1 × 10−6

2

9.3 × 10−3

6.81

4.2 × 10−6

3

8.2 × 10−3

6.86

3.7 × 10−6

Average ± SD

8.13 ± 1.20 × 10−3

6.82 ± 0.04

3.67 ± 0.55 × 10−6

  1. The error rate was calculated using the equation ER = mf/(bp×d), where mf is the mutation frequency that was calculated using the equation mf = white colonies/total colonies, and bp is the number of detectable sites (324) in the lacZ gene of plasmid pUC19, and d is the number of template doublings that were calculated using the equation d = (ng product)/(ng input)
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Microbial Cell Factories

ISSN: 1475-2859

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