Fig. 7

Multiple displacement amplification of pUC19 plasmid by IME199 DNAP. A The primed pUC19 DNA in which the heat-denatured pUC19 plasmid hybridized with random hexamer primers was incubated with 100 nM IME199 DNAP or 10 U phi29 DNAP (NEB, USA) at 30 °C for 2 h, and then analyzed by non-denaturing 1% agarose electrophoresis. B The pUC19 plasmid DNA and multiple displacement amplification of product by IME199 DNAP or phi29 DNAP was digested with SalI-HF for 2 h at 37 °C, and then analyzed by non-denaturing 1% agarose electrophoresis. Experimental details are described in Experimental Procedures