Fig. 2

Exonuclease activity test of IME199 DNAP. Different concentrations of IME199 DNAP were incubated with 400 nM 5′-FAM-labeled 24 nt oligonucleotides (5′FAM-TCCTAACGAGATTAGTTTTGCTGT-3′) (A) or 3′-FAM-labeled 24 nt oligonucleotides (5′-TCCTAACGAGATTAGTTTTGCTGT -FAM3′) (B) at 30 °C for 20 min. The control is the FAM-labeled 24 nt oligonucleotide without IME199 DNAP. No deoxyribonucleoside triphosphates (dNTPs) were added in any of the reactions. All results are presented on 20% denaturing PAGE