Table 1 Primers used for q-RT PCR for intestinal inflammation and integrity
Primer | Forward sequence | Reverse sequence |
|---|---|---|
β-actin | CCAGTTGGTAACAATGCCATGT | GGCTGTATTCCCCTCCATCG |
IL-6 | AAAGAGTTGTGCAATGGCAATTCT | AAGTGCATCATCGTTGTTCATACA |
IL-10 | CGCAGCTCTAGGAGCATGTG | GCTCTTACTGACTGGCATGAG |
TNF-ɑ | AGTGGTGCCAGCCGATGGGTTGT | GCTGAGTTGGTCCCCCTTCTCCAG |
IFN-γ | AGACAATCAGGCCATCAGCA- | AGACAATCAGGCCATCAGCA- |
Occludin | GCTGTGATGTGTGTGAGCTG | GACGGTCTACCTGGAGGAAC |
ZO-1 | AGGACACCAAAGCATGTGAG | GGCATTCCTGCTGGTTACA |
PEDV-M | GGTTCTATTCCCGTTGATGAGGT | AACACAAGAGGCCAAAGTATCCAT |