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Fig. 3 | Microbial Cell Factories

Fig. 3

From: The extracellular polysaccharide inhibit porcine epidemic diarrhea virus with extract and gene editing Lacticaseibacillus

Fig. 3

The genome edition for L. casei genome engineering based on CRISPR-Cas9D10A. A genome editing plasmid deleting the exopolysaccharide biosynthesis gene (glucose-1-phosphate thymidylyltransferase gene, 2179 gene) was constructed in our research in the mechanism diagram (A), The positive genome editing plasmid of L. casei was constructed, and the B shown the diagram losing the glucose-1-phosphate thymidylyltransferase gene, which contain the SgRNA, dCas9, left and right homologous arm genes

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Microbial Cell Factories

ISSN: 1475-2859

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