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Table 3 Kinetic Parameters of iolS and variants toward ONDPAa

From: Semi-rational engineering an aldo–keto reductase for stereocomplementary reduction of α-keto amide compounds

Enzyme

Km (mM)

kcat (s−1)

kcat /Km (s−1·mM−1)

iolS WT

1.81 ± 0.13

3.53 ± 0.12

1.95

I57F

1.72 ± 0.13

3.96 ± 0.23

2.32

F126L

1.55 ± 0.22

3.81 ± 0.13

2.45

N21A

1.16 ± 0.16

4.10 ± 0.12

3.53

I57F/F126L

1.71 ± 0.11

4.32 ± 0.14

2.51

N21A/F126A

1.65 ± 0.12

6.40 ± 0.25

3.88

  1. aNonlinear regression of the kinetic data are shown in Additional file 1: Fig. S2
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Microbial Cell Factories

ISSN: 1475-2859

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