Caproate production from Enset fiber in one-pot two-step fermentation using anaerobic fungi (Neocallimastix cameroonii strain G341) and Clostridium kluyveri DSM 555

Table 1 Carbon balance for N. cameroonii growth on Enset fiber, and two-step fermentation using N. cameroonii and C. kluyveri on Enset fiber

Compound	Carbon per compound (mmol) for N. cameroonii growth on Enset fiber		Carbon per compound (mmol) for two-step fermentation ^a
Compound	Substrates	Products	Substrates	Products
Enset fiber	8.58
Acetate		2.22	2.72	1.69
Ethanol	1.28^b	1.43	2.22	0
Succinate		0.32	0.44	0.24
Lactate		0.49	0.27	0.25
Formate		1.34	1.14	1.11
CO₂	8.06	9.66	9.19	8.32
Caproate				1.65
Butyrate				1.78
Sum of carbon	17.92	15.47	15.97	15.03
Carbon recovery (%)^c	86.3		94.1

^a The two step fermentation was without addition of ethanol; ^b The amount of ethanol came from the hemin solution in the medium; ^c Carbon recovery (%)= (Sum of carbon in products/ Sum of carbon in substrate)×100%

ISSN: 1475-2859