Table 1 Strains and plasmids used in this study

C. glutamicum strains

 DelAro⁴-4cl_Pc C5 mufasO_BCD1 P_O6-iolT1 ∆pyc (M-CoA)

Prophage-free derivative of ATCC13032 with an in-frame deletion of cg0344-47, cg0503, cg2625-40, and cg1226. Chromosomal integration of the codon-optimized gene variant 4cl_PcCg encoding 4-coumarate: CoA ligase from parsley (Petroselinum crispum) (Δcg0344-47:PT7-4cl_PcCg) Exchange of the native promotor of the citrate synthase gene gltA by dapA promotor variant C5. Mutated fasO binding site upstream of accBC and accD1. Two point mutations in the promotor of the inositol transporter gene iolT1 abolish repression of iolT1 by IolR. In-frame deletion of pyc (cg0791) encoding pyruvate carboxylase

[50]

 DelAro⁵ C7 P_O6-iolT1 (p-CA1)

Prophage-free derivative of ATCC13032 with in-frame deletions of cg0344-cg0347, cg2625-cg2640, cg1226, cg502, and cg3349-cg3354 (DelAro⁵). Exchange of the native promotor of the citrate synthase gene gltA by dapA promotor variant C7. Two point mutations in the promotor of the inositol transporter gene iolT1 abolish repression of iolT1 by IolR

[35]

 p-CA1 ∆phdA (p-CA2)

p-CA1 derivative with an in-frame deletion of phdA (cg0341) encoding an endogenous acyl:CoA ligase

This work

 p-CA2 GTG-pheA

p-CA2 derivative with start codon replacement (ATG→GTG) of pheA (cg3207) encoding prephenate dehydratase

This work

 p-CA2 AroF-S188C

p-CA2 derivative harboring the mutated DAHP synthase AroF carrying the amino acid substitution S188C

This work

 p-CA2 GTG-pheA AroF-S188C (p-CA3)

p-CA2 derivative with start codon replacement (ATG→GTG) of pheA (cg3207) encoding prephenate dehydratase and harboring the mutated DAHP synthase AroF carrying the amino acid substitution S188C

This work

 p-CA3 TrpE-P304S (p-CA4)

p-CA3 derivative harboring the mutated anthranilate synthase component I TrpE carrying the amino acid substitution P304S

This work

 p-CA4 ∆phdT (p-CA5)

p-CA4 derivative with an in-frame deletion of phdT (cg0340) encoding the phenylpropanoid transporter PhdT

This work

 p-CA5 ATG-aroK (p-CA6)

p-CA5 derivative with start codon replacement (GTG→ATG) of aroK (cg1828) encoding shikimate kinase

This work

 p-CA6 IGR9::aroF*_EcCg (p-CA7)

p-CA6 derivative with genomic integration of aroF*_EcCg encoding AroF* from E. coli (codon-optimized gene) under control of the dapA promotor variant A16

This work

 p-CA7 ∆pyk (p-CA8)

p-CA7 derivative with an in-frame deletion of pyk (cg2291) encoding pyruvate kinase

This work

E. coli strains

 E. coli DH5α

F^–Φ80lacZΔM15 Δ(lacZYA- argF)U169 recA1 endA1 hsdR17 (rK-, mK+) phoA supE44λ- thi-1 gyrA96 relA1

Invitrogen (Karlsruhe, Germany)

Plasmids

 pMKEx2

kan^r; E. coli-C. glutamicum shuttle vector (lacI, P_T7, lacO1, pHM1519 ori_Cg; pACYC177 ori_Ec)

[70]

 pMKEx2-sts_AhCg-4cl_PcCg

pMKEx2 derivative for expression of sts_AhCg and 4cl_PcCg (codon-optimized genes) encoding stilbene synthase from Arachis hypogaea and 4-coumarate:CoA ligase from Petroselinum crispum

[31]

 pEKEx3

spec^r; E. coli-C. glutamicum shuttle vector (lacI, P_tac, lacO1, pBL1ori_Cg; pUCori_Ec)

[72]

 pEKEx3-aroH_Ec-tal_FjCg

pEKEx3 derivative for expression of aroH_Ec encoding DAHP synthase from E. coli and tal_FjCg (codon-optimized gene) encoding tyrosine ammonia-lyase from Flavobacterium johnsoniae

[31]

 pK19mobsacB

kan^r; vector for allelic exchange in C. glutamicum (pK18 oriVEc sacB lacZα)

[73]

 pK19mobsacB-∆phdA

pK19mobsacB derivative for in-frame deletion of phdA

[37]

 pK19mobsacB-GTG-pheA

pK19mobsacB derivative for the start-codon replacement of pheA (ATG→GTG)

This work

 pK19mobsacB-AroF-S188C

pK19mobsacB derivative for site-directed mutagenesis of aroF encoding DAHP synthase from C. glutamicum resulting in the amino acid substitution S188C

This work

 pK19mobsacB-TrpE-P304S

pK19mobsacB derivative for site-directed mutagenesis of trpE encoding anthranilate synthase component I from C. glutamicum resulting in the amino acid substitution P304S

This work

 pK19mobsacB-∆phdT

pK19mobsacB derivative for in-frame deletion of phdT

[37]

 pK19mobsacB-ATG-aroK

pK19mobsacB derivative for the start-codon replacement of aroK (GTG→ATG)

Mutz et al.(in preparation)

 pK19mobsacB-IGR9::PdapA-A16-aroF*_EcCg

pK19mobsacB-IGR9 derivative for genomic integration of aroF*_EcCg, whose gene expression is under control of the dapA promotor variant A16

Mutz et al.(in preparation)

 pK19mobsacB-∆pyk

pK19mobsacB derivative for in-frame deletion of pyk

[74]