Fig. 4
Effect of amino acid substitution P304S in anthranilate synthase component I (TrpE-P304S) on p-CA production and anthranilate byproduct formation. Growth (OD600) (diamonds), p-CA (circles), and ANT titer (squares) of (a) C. glutamicum p-CA3 and (b) C. glutamicum p-CA4 with mutated anthranilate synthase component I (TrpE-P304S). All strains harbor the expression plasmid pEKEx3-aroHEc-talFjCg for p-CA production. The depicted data represent mean values and standard deviation of biological triplicates