Table 3 Strains used in this study
From: Improvement of ethanol and 2,3-butanediol production in Saccharomyces cerevisiae by ATP wasting
Strain name | Genotype | Source |
|---|---|---|
BY318 | MATa ura3-52 | National BioResource Project |
BY318-Control | BY318 (pGK426) | This study |
BY318-sceFBPase | BY318 (pGK426-sceFBPase) | This study |
BY318-ecoFBPase | BY318 (pGK426-ecoFBPase) | This study |
BY318-opt_ecoFBPase | BY318 (pGK426-opt_ecoFBPase) | This study |
BY318-ATPase | BY318 (pGK426-atpAGD) | This study |
YPH499 | MATa ura3-52 lys2-801 ade2-101 trp1-Δ63 his3-Δ200 leu2-Δ1 | Stratagene / Agilent Technologies |
YSM021 (PDCΔ) | YPH499 pdc1Δ pdc5Δ pdc6Δ MTH1-ΔT(L165F) | Ishii et al. (2018) |
YSM046 (PDCΔ + evolved) | A laboratory-evolved yeast strain derived from PDCΔ (YSM021) strain | Ishii et al. (2018) |
YHI030 | YSM046 (pATP422-alsLpOp-aldcLlOp/pAT425-BDH1) | Ishii et al. (2018) |
YHI030-Control | YHI030 (pGK426) | This study |
YHI030-sceFBPase | YHI030 (pGK426-sceFBPase) | This study |
YHI030-ecoFBPase | YHI030 (pGK426-ecoFBPase) | This study |
YHI030-opt_ecoFBPase | YHI030 (pGK426-opt_ecoFBPase) | This study |
YHI030-ATPase | YHI030 (pGK426-atpAGD) | This study |