Improvement of ethanol and 2,3-butanediol production in Saccharomyces cerevisiae by ATP wasting

Table 2 Growth rates and metabolite consumption and production rates and yields in YHI030 strains

	Specific growth rate (h⁻¹)	Specific glucose consumption rate (mmol/g-DCW/h)	Specific 2,3-butanediol production rate (mmol (mmol/g-DCW/h)	Specific glycerol production rate (mmol (mmol/g-DCW/h)	Specific acetoin production rate (mmol (mmol/g-DCW/h)	2,3-Butanediol yield (mol (mol-glucose)⁻¹)	Glycerol yield (mol (mol-glucose)⁻¹)	Acetoin yield (mol (mol-glucose)⁻¹)
Control	0.044 ± 0.000	0.920 ± 0.014	0.464 ± 0.017	0.316 ± 0.015	0.140 ± 0.020	0.505 ± 0.011	0.344 ± 0.022	0.152 ± 0.022
Scefbpase	0.044 ± 0.001	0.824 ± 0.019**	0.436 ± 0.015	0.319 ± 0.024	0.168 ± 0.018	0.529 ± 0.009*	0.388 ± 0.037	0.204 ± 0.025
Ecofbpase	0.043 ± 0.001	0.911 ± 0.017	0.436 ± 0.024	0.323 ± 0.007	0.133 ± 0.007	0.478 ± 0.025	0.355 ± 0.010	0.146 ± 0.006
Opt_ecoFBPase	0.036 ± 0.001***	0.797 ± 0.043**	0.506 ± 0.028	0.317 ± 0.041	0.164 ± 0.019	0.637 ± 0.059*	0.400 ± 0.068	0.207 ± 0.034
ATPase	0.042 ± 0.002	1.06 ± 0.06*	0.633 ± 0.029***	0.419 ± 0.002***	0.181 ± 0.023	0.598 ± 0.020**	0.397 ± 0.025*	0.170 ± 0.017

Asterisks indicate significant differences by two-tailed Student’s t-test, versus control. *p < 0.05, **p < 0.01, ***p < 0.001. The dry cell weight was set at 0.28
Specific rates and yields were determined from the data over a period of 0–48 h and 24–72 h, respectively

ISSN: 1475-2859