Improvement of ethanol and 2,3-butanediol production in Saccharomyces cerevisiae by ATP wasting

Table 1 Growth rate and metabolite consumption and production rates and yields of BY318 strains

	Specific growth rate (h⁻¹)	Specific glucose consumption rate (mmol/g-DCW/h)	Specific glycerol production rate (mmol (mmol/g-DCW/h)	Specific ethanol production rate (mmol (mmol/g-DCW/h)	Specific acetate production rate (mmol (mmol/g-DCW/h)	Glycerol yield (mol (mol-glucose)⁻¹)	Ethanol yield (mol (mol-glucose)⁻¹)	Acetate yield (mol (mol-glucose)⁻¹)
control	0.304 ± 0.000	8.05 ± 0.42	1.11 ± 0.03	14.0 ± 0.4	0.242 ± 0.019	0.138 ± 0.006	1.75 ± 0.09	0.030 ± 0.002
sceFBPase	0.282 ± 0.005**	9.10 ± 1.55	1.30 ± 0.14	14.4 ± 1.2	0.351 ± 0.017**	0.145 ± 0.019	1.60 ± 0.17	0.039 ± 0.005*
ecoFBPase	0.302 ± 0.005	8.29 ± 1.55	1.08 ± 0.02	14.6 ± 1.4	0.247 ± 0.027	0.133 ± 0.022	1.78 ± 0.15	0.031 ± 0.008
opt_ecoFBPase	0.302 ± 0.000**	10.5 ± 0.3**	1.31 ± 0.02***	17.4 ± 0.6**	0.266 ± 0.008	0.125 ± 0.002*	1.66 ± 0.03	0.025 ± 0.001**
ATPase	0.275 ± 0.000***	11.4 ± 1.0**	1.19 ± 0.00**	20.3 ± 0.5***	0.286 ± 0.013*	0.105 ± 0.009**	1.79 ± 0.11	0.025 ± 0.001**

Asterisks indicate significant differences by two-tailed Student’s t-test, versus control. *p < 0.05, **p < 0.01, ***p < 0.001. The dry cell weight was set to 0.28. All specific rates and yields were determined from the data over a period of 3–12 h

ISSN: 1475-2859