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Table 1 Design of Media for Growth on Hexadecane as Sole Carbon Source*

From: Two-step conversion of polyethylene into recombinant proteins using a microbial platform

Hexadecane

Ammonium Chloride, NH4Cl

Ammonium Nitrate, NH4NO3

0.46% w/v

or

4.6% w/v

or

9.2% w/v

2.5 g/L (0.65 g/L elemental N)

or

5 g/L (1.3 g/L elemental N)

1.9 g/L (0.65 g/L elemental N)

or

3.8 g/L (1.3 g/L elemental N)

  1. *The minimal salt media background maintains a constant composition for all conditions