Fig. 3

Titers of recombinant GFPuv when strain P. aeruginosa RR1 g-GFPuv is grown on hexadecane under a variety of culture conditions. a Cultures grown with either 2.5 or 5 g/L of NH₄Cl (2.5 Cl and 5 Cl). b Cultures grown with either 1.9 or 3.8 g/L of NH₄NO₃ (1.9 NO₃ and 3.8 NO₃). Error bars represent standard deviations from the mean value of three replicates. 0.3 mM IPTG was added at 24, 48, or 72 h post inoculation. Total culturing time was 96 h, resulting in expression times of either 72, 48, or 24 h. Hexadecane concentrations (C16) are provided as % w/v