Fig. 3

(a) Metabolic pathways of S. coelicolor M1152 that present gene manipulation targets predicted using the genome-scale metabolic model ScoSBML2119. Locus tags in red indicate overexpression targets. Reaction IDs are also presented in boxes for each gene manipulation target. Enzyme names for the presented reaction IDs are: PSERT, phosphoserine transaminase; MTHFD, methylenetetrahydrofolate dehydrogenase; and ACCOAC, acetyl-CoA carboxylase. (b) Analysis of oviedomycin production in SCMO1F with individual overexpression of the three target genes predicted using the GEM. In the table, ‘O’ and ‘-’ denote inclusion and absence of an overexpressed gene predicted using the GEM, respectively. Data represent the average and standard deviation of three independent experiments