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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Development of a counterselectable system for rapid and efficient CRISPR-based genome engineering in Zymomonas mobilis

Fig. 2

Plasmid curing using the clmPheS as a counterselection. (A) Transformation efficiencies of the shuttle vector pEZ15Asp and the clmPheS-expressing plasmid pSsp-RFP. Three replicates were performed for each DNA sample. (B) Growth curve measurements of the pEZ15Asp and pSsp-RFP transformants. Three replicates were performed for the experiment. (C) Examination of the counterselection effect of 4-CP on plasmid curing from the pSsp-RFP transformant. (D) Detection of RFP signal in cells of the pEZ15Asp transformant, and that of the pSsp-RFP transformant before and after 4-CP counterselection

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Microbial Cell Factories

ISSN: 1475-2859

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