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Fig. 1 | Microbial Cell Factories

Fig. 1

From: Development of a counterselectable system for rapid and efficient CRISPR-based genome engineering in Zymomonas mobilis

Fig. 1

Construction of the clmPheS counterselection marker. (A) Multiple sequence alignment of PheS orthologs derived from Zymobilis mobilis ZM4 (ZmoPheS), Escherichia coli K12 (EcoPheS), Lactococcus lactis MG1363 (LacPheS), Staphylococcus aureus MW2 (SauPheS), Bacillus subtilis 168 (BsuPheS), and Methylotuvimicrobium alcaliphilum 20Z (MalPheS). Red boxes indicate the conserved residuals being subjected to mutagenesis. (B) 4-CP sensitivity of Z. mobilis transformants. Cell cultures of transformants either harboring the shuttle vector pEZ15Asp, or the clmPheS-expressing plasmid pSsp, were serially 10-fold diluted. Dilutions were spotted onto agar plates containing 4-CP at the indicated concentrations

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Microbial Cell Factories

ISSN: 1475-2859

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