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Table 4 Box–Behnken design for the production of EPS-BP5M

From: Statistical optimization of waste molasses-based exopolysaccharides and self-sustainable bioelectricity production for dual chamber microbial fuel cell by Bacillus piscis

Run

F1

F2

F3

Actual value

Predicted value

A, molasses (X1) %

B, MgSO4 (X2) g/L

C, inoculum size (X3) %

EPS-BP5M Yield (g/L)

1

8

0.5

2

18.27

17.71

2

8

0.2

4

12.91

13.90

3

5

0.5

4

37.11

37.11

4

5

0.2

2

4.10

3.67

5

5

0.5

4

37.11

37.11

6

8

0.8

4

14.86

15.39

7

8

0.5

6

21.83

20.87

8

2

0.5

6

9.45

10.01

9

5

0.8

6

7.28

7.71

10

5

0.5

4

37.11

37.11

11

2

0.5

2

6.10

7.06

12

5

0.5

4

37.11

37.11

13

2

0.2

4

4.17

3.64

14

2

0.8

4

5.10

4.12

15

5

0.8

2

5.99

6.02

16

5

0.5

4

37.11

37.11

17

5

0.2

6

8.12

8.09

  1. F factor