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Table 2 Optimization of EPS-BP5M production from B. piscis in molasses-based medium by PBD

From: Statistical optimization of waste molasses-based exopolysaccharides and self-sustainable bioelectricity production for dual chamber microbial fuel cell by Bacillus piscis

Run

F1

F2

F3

F4

F 5

F6

F7

F8

F 9

F10

F 11

Response ( EPS-BP5M Yield)

A:

Molasses, (X1)

B:

Yeast

ex., (X2)

C:

Peptone

ex., (X3)

D:

NaCl, (X4)

E:

KH2PO4, (X5)

F:

K2HPO4, (X6)

G:

NaNO3, (X7)

H:

MgSO4, (X8)

J:

MgCl, (X9)

K:

Inoculum size, (X10)

L:

pH, (X11)

Actual

value

Predicted

value

%

g/L

g/L

g/L

g/L

g/L

g/L

g/L

g/L

%

pH

g/L

1

8

2

2

0.5

0.3

1

5.0

0.8

0.5

6

8

27.5

27.26

2

2

2

2

0.5

0.1

1

2.5

0.2

0.5

2

6

14.05

13.86

3

8

4

2

0.5

0.1

2

2.5

0.8

2.5

2

8

23.88

24.20

4

2

2

2

1.0

0.1

2

5.0

0.2

2.5

6

8

12.12

11.68

5

8

4

2

1.0

0.3

2

2.5

0.2

0.5

6

6

24.98

26.00

6

8

2

4

1.0

0.3

1

2.5

0.2

2.5

2

8

18.63

19.07

7

2

4

4

1.0

0.1

1

2.5

0.8

0.5

6

8

17.22

16.95

8

2

4

2

1.0

0.3

1

5.0

0.8

2.5

2

6

14.3

14.06

9

2

2

4

0.5

0.3

2

2.5

0.8

2.5

6

6

16.05

16.99

10

8

2

4

1.0

0.1

2

5.0

0.8

0.5

2

6

25.03

24.51

11

8

4

4

0.5

0.1

1

5.0

0.2

2.5

6

6

26.89

25.87

12

2

4

4

0.5

0.3

2

5.0

0.2

0.5

2

8

13.5

13.69

  1. F factor
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Microbial Cell Factories

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