CRISPR-Cas9 assisted non-homologous end joining genome editing system of Halomonas bluephagenesis for large DNA fragment deletion

Table 4 The deletion results of 19–50 kb fragments

Deletion fragment	Delete length (bp)	Colony number	Deletion efficiency
19 kb (36,955–56,496 bp)^a	Wild type	42	12.5%
19 kb (36,955–56,496 bp)^a	19,000–23,000	6	12.5%
26 kb (692,569–718,967 bp)	Wild type	30	37.5%
26 kb (692,569–718,967 bp)	26,000–30,000	18	37.5%
50 kb (2,138,365–2,192,787 bp)	Wild type	33	31.3%
50 kb (2,138,365–2,192,787 bp)	50,000–55,000	15	31.3%

ISSN: 1475-2859