Table 3 The deletion results of 4–18 kb fragments
Deletion fragment | Delete length (bp) | Colony number | Deletion efficiency |
|---|---|---|---|
4 kb (52,204–56,496 bp) | Wild type | 19 | 81% |
3000–5000 | 24 | ||
5000–7000 | 18 | ||
7000–10,000 | 9 | ||
10,000–15,000 | 20 | ||
15,000–20,000 | 7 | ||
>20,000 | 3 | ||
7 kb (49,199–56,507 bp) | Wild type | 26 | 74% |
<6000 | 1 | ||
6000–8000 | 30 | ||
8000–10,000 | 23 | ||
10,000–15,000 | 8 | ||
15,000–20,000 | 12 | ||
9 kb (49,407–58,544 bp) | Wild type | 13 | 87% |
7000–9000 | 8 | ||
9000–10,000 | 35 | ||
10,000–15,000 | 23 | ||
15,000–20,000 | 19 | ||
>20,000 | 2 | ||
15 kb (41,405–56,496 bp) | Wild type | 96 | 4% |
|  | 15,000–17,000 | 4 | |
18 kb (38,533–56,496 bp) | Wild type | 98 | 2% |
|  | 18,000–19,000 | 2 | |
18 kb (38,533–56,496 bp)a | Wild type | 86 | 14% |
18,000–20,000 | 14 |