CRISPR-Cas9 assisted non-homologous end joining genome editing system of Halomonas bluephagenesis for large DNA fragment deletion

Table 2 Results of gene deletion guided by single sgRNA

Target gene (length)	Average deletion efficiency	Delete length (bp)	Insert length (bp)
phaC (1851 bp)	12.3 ± 2.5%	45–1107	51–87
gabD3 (1446 bp)	6.3 ± 1.5%	86–901	30–55
rfbD1 (897 bp)	10.0 ± 2.0%	13–519	29–144
rfbD2 (891 bp)	33.3 ± 5.5%	22–632	2–563
TD01-01921-23 (2484 bp)	7.7 ± 0.6%	10–2281	/
TD01-00059-61 (2961 bp)	24.0 ± 1.0%	1077–3887^a	14–176

^a TD01-00059-61 is a repeat sequence and the selected sgRNA has two matches in this gene

ISSN: 1475-2859