Skip to main content
Fig. 4 | Microbial Cell Factories

Fig. 4

From: CRISPR-Cas9 assisted non-homologous end joining genome editing system of Halomonas bluephagenesis for large DNA fragment deletion

Fig. 4

Optimization of Cas9 expression in H. bluephagenesis TD01. (A) The GFP fluorescence intensity induced by different concentrations of arabinose. (B) Deletion efficiency of phaC gene by two sgRNAs when Cas9 protein was induced by Para under different arabinose concentration. For each experiment of phaC deletion efficiency determination, fifty clones were randomly selected for colony PCR and all experiments were performed in triplicates. The deletion efficiency was calculated by dividing the number of positive deletion clones by the total number of selected clones

Back to article page

Microbial Cell Factories

ISSN: 1475-2859

Contact us